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验证数据展示

  • WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

    WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67500-1-Ig (RAN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAN antibody included
  • IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RAN antibody (67500-1-Ig, Clone: 1B11E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

RAN Monoclonal antibody, PBS Only

RAN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67500-1-PBS

产品说明书

CloneNo. 1B11E1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.

产品信息

67500-1-PBS targets RAN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 RAN fusion protein Ag30034 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RAN, member RAS oncogene family
别名 ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4
计算分子量 25 kDa
观测分子量 24 kDa
GenBank蛋白编号BC004272
基因名称 RAN
Gene ID (NCBI) 5901
RRIDAB_2882724
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62826
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).