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RAN Monoclonal antibody, PBS Only

RAN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11E1

Cat no : 67500-1-PBS

Print datasheet

Synonyms

ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4

验证数据展示

  • WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

    WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67500-1-Ig (RAN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RAN antibody (67500-1-Ig, Clone: 1B11E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

产品信息

67500-1-PBS targets RAN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RAN fusion protein Ag30034 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name RAN, member RAS oncogene family
Synonyms ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC004272
Gene Symbol RAN
Gene ID (NCBI) 5901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62826
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).