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RANBP3 Polyclonal antibody

RANBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27168-1-AP

Print datasheet

Synonyms

Ran-binding protein 3, RAN binding protein 3

验证数据展示

  • WB analysis using 27168-1-AP

    WB analysis using 27168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27168-1-AP (RANBP3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 27168-1-AP

    IHC staining of mouse testis using 27168-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27168-1-AP (RANBP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 27168-1-AP

    IF Staining of HepG2 using 27168-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RANBP3 antibody (27168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27168-1-AP targets RANBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RANBP3 fusion protein Ag26000 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAN binding protein 3
Synonyms Ran-binding protein 3, RAN binding protein 3
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC004349
Gene Symbol RANBP3
Gene ID (NCBI) 8498
RRIDAB_3669586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6Z4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RANBP3 (Ran-binding protein 3) is also named as Rab escort protein 1 (REP-1). RanBP3 is a RanGTP‐binding protein that has been reported to enhance nuclear export of active β‐catenin, independent of its role as a CRM1‐associated nuclear export cofactorl (PMID: 9637251). RanBP3 has been reported to be a crucial downstream molecule of MAPK-ERK and PI3K-AKT signal pathways (PMID: 18331709). RanBP3 is a tumor suppressor in CRC and is associated with patient survival (PMID: 36270974). RanBP3 has been reported as a potential regulator in melanoma biology, hepatocellular carcinoma, prostate cancer, and ovarian cancer, and RanBP3 is highly expressed in CML Cells (PMID: 34504783).

实验方案

Product Specific Protocols
WB protocol for RANBP3 antibody 27168-1-APDownload protocol
IHC protocol for RANBP3 antibody 27168-1-APDownload protocol
IF protocol for RANBP3 antibody 27168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols