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RANBP3L Polyclonal antibody

RANBP3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17875-1-AP

Print datasheet

Synonyms

RAN binding protein 3 like, RANBP3L

验证数据展示

  • WB analysis using 17875-1-AP

    WB analysis using 17875-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 17875-1-AP

    IHC staining of mouse kidney using 17875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 17875-1-AP

    IHC staining of mouse kidney using 17875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17875-1-AP targets RANBP3L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RANBP3L fusion protein Ag11642 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAN binding protein 3-like
Synonyms RAN binding protein 3 like, RANBP3L
Calculated Molecular Weight 465 aa, 52 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC047660
Gene Symbol RANBP3L
Gene ID (NCBI) 202151
RRIDAB_2284894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RANBP3L antibody 17875-1-APDownload protocol
IHC protocol for RANBP3L antibody 17875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols