验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse testis |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human skin tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RANBP9 Ready-To-Use IHC Kit. RANBP9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17755-1-AP targets RANBP9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | RANBP9 fusion protein Ag12112 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAN binding protein 9 |
| 别名 | RanBP7, Ran-binding protein M, Ran-binding protein 9, BPM-L, BPM90 |
| 计算分子量 | 388aa,43 kDa; 729aa,78 kDa |
| 观测分子量 | 80-90 kDa |
| GenBank蛋白编号 | BC063849 |
| 基因名称 | RANBP9 |
| Gene ID (NCBI) | 10048 |
| RRID | AB_2176803 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96S59 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RANBP9 antibody 17755-1-AP | Download protocol |
| IHC protocol for RANBP9 antibody 17755-1-AP | Download protocol |
| IF protocol for RANBP9 antibody 17755-1-AP | Download protocol |
| IP protocol for RANBP9 antibody 17755-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
mSphere B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition. | ||
Acta Histochem MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9. | ||
Transl Cancer Res Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients. | ||
Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth |
