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验证数据展示

  • WB analysis of HeLa using 17755-1-AP

    WB analysis of HeLa using 17755-1-AP

    WB result of RANBP9 antibody (17755-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANBP9 transfected HeLa cells.

  • WB analysis using 17755-1-AP

    WB analysis using 17755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17755-1-AP (RANBP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17755-1-AP

    IHC staining of human testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RANBP9 antibody included
  • IHC staining of human testis using 17755-1-AP

    IHC staining of human testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with RANBP9 antibody included
  • IP experiment of HeLa using 17755-1-AP

    IP experiment of HeLa using 17755-1-AP

    IP result of anti-RANBP9 (IP:17755-1-AP, 4ug; Detection:17755-1-AP 1:600) with HeLa cells lysate 1600ug.

  • IF Staining of HeLa using 17755-1-AP

    IF Staining of HeLa using 17755-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RANBP9 antibody (17755-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Featured Product
  • KD/KO Validated

RANBP9 Polyclonal antibody

RANBP9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17755-1-AP

Print datasheet

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Synonyms

RanBP7, Ran-binding protein M, Ran-binding protein 9, BPM-L, BPM90

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, mouse testis
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human skin tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RANBP9 Ready-To-Use IHC Kit. RANBP9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17755-1-AP targets RANBP9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat
Immunogen RANBP9 fusion protein Ag12112 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAN binding protein 9
Synonyms RanBP7, Ran-binding protein M, Ran-binding protein 9, BPM-L, BPM90
Calculated Molecular Weight388aa,43 kDa; 729aa,78 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC063849
Gene Symbol RANBP9
Gene ID (NCBI) 10048
RRIDAB_2176803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96S59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RANBP9 antibody 17755-1-APDownload protocol
IHC protocol for RANBP9 antibody 17755-1-APDownload protocol
IF protocol for RANBP9 antibody 17755-1-APDownload protocol
IP protocol for RANBP9 antibody 17755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

mSphere

B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition.

Authors - Yan Chen
View Article
humanWB

Oncol Lett

RANBP9 suppresses tumor proliferation in colorectal cancer.

Authors - Chunzhi Qin
View Article
ratIF

Acta Histochem

MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9.

Authors - Huiyuan Zhang
View Article
humanIHC

Transl Cancer Res

Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients.

Authors - Guangbin Wu
View Article
mouseWB

Nat Commun

Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth

Authors - Nelson V Simwela
View Article