- Featured Product
- KD/KO Validated
RANGAP1 Polyclonal antibody
RANGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11102-1-AP targets RANGAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IP, CoIP, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | RANGAP1 fusion protein Ag1563 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Ran GTPase activating protein 1 |
| Synonyms | SD, Ran GTPase-activating protein 1, KIAA1835 |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC014044 |
| Gene Symbol | RANGAP1 |
| Gene ID (NCBI) | 5905 |
| RRID | AB_2176968 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46060 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RANGAP1 antibody 11102-1-AP | Download protocol |
| IHC protocol for RANGAP1 antibody 11102-1-AP | Download protocol |
| IF protocol for RANGAP1 antibody 11102-1-AP | Download protocol |
| IP protocol for RANGAP1 antibody 11102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis | ||
J Proteomics mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances. | ||
Dev Cell Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma | ||
Cancer Gene Ther METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1
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