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RanGAP1 Polyclonal antibody

RanGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11102-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

KIAA1835, Ran GTPase-activating protein 1, SD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11102-1-AP targets RanGAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 RanGAP1 fusion protein Ag1563 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ran GTPase activating protein 1
别名 KIAA1835, Ran GTPase-activating protein 1, SD
计算分子量 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC014044
基因名称 RANGAP1
Gene ID (NCBI) 5905
RRIDAB_2176968
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46060
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RanGAP1 antibody 11102-1-APDownload protocol
IHC protocol for RanGAP1 antibody 11102-1-APDownload protocol
IF protocol for RanGAP1 antibody 11102-1-APDownload protocol
IP protocol for RanGAP1 antibody 11102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,CoIP

Cell Oncol (Dordr)

Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis

Authors - Hong-Juan You
humanWB

J Proteomics

mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances.

Authors - Xianwei Chen
human,mouseWB,IHC,IP

Dev Cell

Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma

Authors - Yan Gong
humanWB,IHC,IP

Cancer Gene Ther

METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1

Authors - Rui Yang
  • KD Validated
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