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RANGAP1 Monoclonal antibody, PBS Only

RANGAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3A4

Cat No : 67146-1-PBS

Print datasheet

Synonyms

SD, Ran GTPase-activating protein 1, KIAA1835, 1F3A4

验证数据展示

  • WB analysis of HeLa using 67146-1-Ig (same clone as 67146-1-PBS)

    WB analysis of HeLa using 67146-1-Ig (same clone as 67146-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • WB analysis using 67146-1-Ig (same clone as 67146-1-PBS)

    WB analysis using 67146-1-Ig (same clone as 67146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67146-1-Ig (same clone as 67146-1-PBS)

    IF Staining of HepG2 using 67146-1-Ig (same clone as 67146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67146-1-Ig (RANGAP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

产品信息

67146-1-PBS targets RANGAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RANGAP1 fusion protein Ag26136 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Ran GTPase activating protein 1
Synonyms SD, Ran GTPase-activating protein 1, KIAA1835, 1F3A4
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64 kDa, 82 kDa
GenBank Accession NumberBC014044
Gene Symbol RANGAP1
Gene ID (NCBI) 5905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46060
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.