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RANGRF Polyclonal antibody

RANGRF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10552-1-AP

Print datasheet

Synonyms

RanGNRF, Ran-binding protein MOG1

验证数据展示

  • WB analysis of SGC-7901 using 10552-1-AP

    WB analysis of SGC-7901 using 10552-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 10552-1-AP

    IHC staining of human colon cancer using 10552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10552-1-AP

    IHC staining of human colon cancer using 10552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 10552-1-AP

    IF Staining of HepG2 using 10552-1-AP

    Immunofluorescent analysis of HepG2 cells using 10552-1-AP (RANGRF antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSGC-7901 cells, HepG2 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

10552-1-AP targets RANGRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen RANGRF fusion protein Ag0824 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAN guanine nucleotide release factor
Synonyms RanGNRF, Ran-binding protein MOG1
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18-22 kDa
GenBank Accession NumberBC006486
Gene Symbol RANGRF
Gene ID (NCBI) 29098
RRIDAB_2238173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HD47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RANGRF, also named as MOG1, RANGNRF, HSPC165, HSPC236 and MDS5, regulates the intracellular trafficking of RAN. In cardiac cells it regulates the cell surface localization of SCN5A.

实验方案

Product Specific Protocols
WB protocol for RANGRF antibody 10552-1-APDownload protocol
IHC protocol for RANGRF antibody 10552-1-APDownload protocol
IF protocol for RANGRF antibody 10552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Sci Transl Med

Gene therapy targeting protein trafficking regulator MOG1 in mouse models of Brugada syndrome, arrhythmias, and mild cardiomyopathy.

Authors - Gang Yu
View Article

Stem Cell Res

Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system

Authors - Meiling Jiang
View Article