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验证数据展示

  • IHC staining of human liver cancer using 68125-1-Ig (same clone as 68125-1-PBS)

    IHC staining of human liver cancer using 68125-1-Ig (same clone as 68125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68125-1-PBS in a different storage buffer formulation.

  • WB analysis using 68125-1-Ig (same clone as 68125-1-PBS)

    WB analysis using 68125-1-Ig (same clone as 68125-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68125-1-Ig (RAP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68125-1-PBS in a different storage buffer formulation.

RAP1A Monoclonal antibody, PBS Only

RAP1A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68125-1-PBS

产品说明书

CloneNo. 1G12D8

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

C21KG, G 22K, GTP binding protein smg p21A, KREV 1, KREV1, RAP1, RAP1A, Ras related protein Krev 1, Ras related protein Rap 1A, SMGP21

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68125-1-PBS targets RAP1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 RAP1A fusion protein Ag9463 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAP1A, member of RAS oncogene family
别名 C21KG, G 22K, GTP binding protein smg p21A, KREV 1, KREV1, RAP1, RAP1A, Ras related protein Krev 1, Ras related protein Rap 1A, SMGP21
计算分子量 184 aa, 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC014086
基因名称 RAP1A
Gene ID (NCBI) 5906
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62834
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).