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RAP1B Polyclonal antibody

RAP1B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10840-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, ELISA and More (2)

GTP binding protein smg p21B, K REV, RAL1B, RAP1, RAP1B, Ras related protein Rap 1b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, A549 cells, C6 cells, A431 cells, human brain tissue, HepG2 cells, mouse kidney tissue, NIH/3T3 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAP1B Ready-To-Use IHC Kit. RAP1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10840-1-AP targets RAP1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, chicken
免疫原 RAP1B fusion protein Ag1282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAP1B, member of RAS oncogene family
别名 GTP binding protein smg p21B, K REV, RAL1B, RAP1, RAP1B, Ras related protein Rap 1b
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC000176
基因名称 RAP1B
Gene ID (NCBI) 5908
RRIDAB_2253539
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61224
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

实验方案

Product Specific Protocols
WB protocol for RAP1B antibody 10840-1-APDownload protocol
IHC protocol for RAP1B antibody 10840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis.

Authors - Yajuan Zhang
humanWB

Oncotarget

LncRNA MT1JP functions as a tumor suppressor by interacting with TIAR to modulate the p53 pathway.

Authors - Lihui Liu
humanWB

Int J Mol Sci

Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B.

Authors - Sonja Hinz
chickenWB,IP

Microbiol Spectr

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Authors - Beibei Zhang
humanWB

Am J Cancer Res

Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B

Authors - Jiao Liu
humanWB,IF

Viruses

ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells

Authors - Beibei Zhang
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