验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse brain tissue, A549 cells, C6 cells, A431 cells, human brain tissue, HepG2 cells, mouse kidney tissue, NIH/3T3 cells |
Positive IHC detected in | human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10840-1-AP targets RAP1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, chicken |
免疫原 | RAP1B fusion protein Ag1282 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAP1B, member of RAS oncogene family |
别名 | GTP binding protein smg p21B, K REV, RAL1B, RAP1, RAP1B, Ras related protein Rap 1b |
计算分子量 | 21 kDa |
观测分子量 | 21 kDa |
GenBank蛋白编号 | BC000176 |
基因名称 | RAP1B |
Gene ID (NCBI) | 5908 |
RRID | AB_2253539 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P61224 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.
实验方案
Product Specific Protocols | |
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WB protocol for RAP1B antibody 10840-1-AP | Download protocol |
IHC protocol for RAP1B antibody 10840-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis. | ||
Oncotarget LncRNA MT1JP functions as a tumor suppressor by interacting with TIAR to modulate the p53 pathway. | ||
Int J Mol Sci Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B. | ||
Microbiol Spectr Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization. | ||
Am J Cancer Res Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B | ||
Viruses ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells |