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RAP1GAP Polyclonal antibody

RAP1GAP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19174-1-AP

Print datasheet

Synonyms

KIAA0474, RAP1 GTPase activating protein, RAP1GA1, RAP1GAP, RAP1GAP1, RAP1GAPII, RAPGAP

验证数据展示

  • WB analysis using 19174-1-AP

    WB analysis using 19174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 19174-1-AP

    IF Staining of HeLa using 19174-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAP1GAP antibody (19174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19174-1-AP targets RAP1GAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAP1GAP fusion protein Ag5576 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAP1 GTPase activating protein
Synonyms KIAA0474, RAP1 GTPase activating protein, RAP1GA1, RAP1GAP, RAP1GAP1, RAP1GAPII, RAPGAP
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC054490
Gene Symbol RAP1GAP
Gene ID (NCBI) 5909
RRIDAB_2177291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAP1GAP antibody 19174-1-APDownload protocol
IF protocol for RAP1GAP antibody 19174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols