RAP1GDS1 Polyclonal antibody

RAP1GDS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10377-1-AP

Print datasheet

Synonyms

Exchange factor smgGDS, GDS1, RAP1GDS1, SMG GDS protein, SmgGDS



经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAP1GDS1 Ready-To-Use IHC Kit. RAP1GDS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10377-1-AP targets RAP1GDS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, chicken
Immunogen RAP1GDS1 fusion protein Ag0230 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAP1, GTP-GDP dissociation stimulator 1
Synonyms Exchange factor smgGDS, GDS1, RAP1GDS1, SMG GDS protein, SmgGDS
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC001851
Gene Symbol RAP1GDS1
Gene ID (NCBI) 5910
RRIDAB_2284927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDS stimulates the GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins), including at least c-Ki-ras p21, smg p21A, smg p21B, rhoA p21 and rhoB p21, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the control of integrin-mediated cell adhesion. RAP1GDS1 (synonym: GDS1)is one of RAP1 GTP-GDP dissociation stimulators.

实验方案

Product Specific Protocols
WB protocol for RAP1GDS1 antibody 10377-1-APDownload protocol
IHC protocol for RAP1GDS1 antibody 10377-1-APDownload protocol
IP protocol for RAP1GDS1 antibody 10377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IP

Nat Genet

Mutations in RABL3 alter KRAS prenylation and are associated with hereditary pancreatic cancer.

Authors - Sahar Nissim
chickenWB,IF

Microbiol Spectr

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Authors - Beibei Zhang
humanWB

Oncol Lett

SUMO4 small interfering RNA attenuates invasion and migration via the JAK2/STAT3 pathway in non-small cell lung cancer cells.

Authors - Jin-Xiao Liang
human,mouseWB

Commun Biol

Vimar/RAP1GDS1 promotes acceleration of brain aging after flies and mice reach middle age

Authors - Ying Xiong