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RAP2A Monoclonal antibody, PBS Only

RAP2A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F6F3

Cat no : 68368-1-PBS

Print datasheet

Synonyms

RbBP 30, Ras-related protein Rap-2a, Ras related protein Rap 2a, RAP2, EC:3.6.5.2

验证数据展示

  • WB analysis using 68368-1-Ig (same clone as 68368-1-PBS)

    WB analysis using 68368-1-Ig (same clone as 68368-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68368-1-Ig (RAP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68368-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68368-1-Ig (same clone as 68368-1-PBS)

    IF Staining of MCF-7 using 68368-1-Ig (same clone as 68368-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAP2A antibody (68368-1-Ig, Clone: 1F6F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68368-1-PBS in a different storage buffer formulation.

产品信息

68368-1-PBS targets RAP2A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen RAP2A fusion protein Ag4979 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAP2A, member of RAS oncogene family
Synonyms RbBP 30, Ras-related protein Rap-2a, Ras related protein Rap 2a, RAP2, EC:3.6.5.2
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC041333
Gene Symbol RAP2A
Gene ID (NCBI) 5911
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10114
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras-related protein Rap-2a (RAP2A) is also named as RbBP-30, and belongs to the small GTPase superfamily and Ras family. RAP2A is small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form, RAP2A can interact with and regulates several effectors including MAP4K4, MINK1 and TNIK. a Nedd4-1/Rap2A/TNIK signaling pathway regulates neurite growth and arborization in mammalian neurons (PMID:20159449). Early research indicated that RAP2A is mainly located in the Golgi apparatus of mammalian cells. RAP2A functions in several cascade signals to regulate biological behaviors such as cytoskeletal rearrangement, cell growth and apoptosis, and cell adhesion and migration (PMID:34018090, PMID:16246175, PMID: 16540189).