RAP2B Polyclonal antibody

RAP2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16266-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

Ras-related protein Rap-2b, Ras related protein Rap 2b, EC:3.6.5.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue, HEK-293 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16266-1-AP targets RAP2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 RAP2B fusion protein Ag9331 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAP2B, member of RAS oncogene family
别名 Ras-related protein Rap-2b, Ras related protein Rap 2b, EC:3.6.5.2
计算分子量 183 aa, 21 kDa
观测分子量 19-22 kDa
GenBank蛋白编号BC012362
基因名称 RAP2B
Gene ID (NCBI) 5912
RRIDAB_2177311
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61225
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAP2B belongs to a family of RAS-related genes. It share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. Rap2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.

实验方案

Product Specific Protocols
WB protocol for RAP2B antibody 16266-1-APDownload protocol
IHC protocol for RAP2B antibody 16266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Biol Chem

DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.

Authors - Li Yi Y
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