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验证数据展示

  • WB analysis using 16266-1-AP

    WB analysis using 16266-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 16266-1-AP

    IHC staining of human stomach using 16266-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16266-1-AP (RAP2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAP2B Polyclonal antibody

RAP2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16266-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ras-related protein Rap-2b, Ras related protein Rap 2b, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue, HEK-293 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16266-1-AP targets RAP2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RAP2B fusion protein Ag9331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAP2B, member of RAS oncogene family
Synonyms Ras-related protein Rap-2b, Ras related protein Rap 2b, EC:3.6.5.2
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC012362
Gene Symbol RAP2B
Gene ID (NCBI) 5912
RRIDAB_2177311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61225
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAP2B belongs to a family of RAS-related genes. It share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. Rap2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.

实验方案

Product Specific Protocols
WB protocol for RAP2B antibody 16266-1-APDownload protocol
IHC protocol for RAP2B antibody 16266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Biol Chem

DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.

Authors - Li Yi Y
View Article