验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, MCF-7 cells, rat brain tissue, HEK-293 cells |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
16266-1-AP targets RAP2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | RAP2B fusion protein Ag9331 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAP2B, member of RAS oncogene family |
别名 | Ras-related protein Rap-2b, Ras related protein Rap 2b, EC:3.6.5.2 |
计算分子量 | 183 aa, 21 kDa |
观测分子量 | 19-22 kDa |
GenBank蛋白编号 | BC012362 |
基因名称 | RAP2B |
Gene ID (NCBI) | 5912 |
RRID | AB_2177311 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P61225 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAP2B belongs to a family of RAS-related genes. It share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. Rap2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.
实验方案
Product Specific Protocols | |
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WB protocol for RAP2B antibody 16266-1-AP | Download protocol |
IHC protocol for RAP2B antibody 16266-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |