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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 31299-1-AP

    WB analysis using 31299-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31299-1-AP (RAPGEF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 31299-1-AP

    IF Staining of mouse kidney using 31299-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using RAPGEF2 antibody (31299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAPGEF2 Polyclonal antibody

RAPGEF2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 31299-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

PDZGEF1, PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31299-1-AP targets RAPGEF2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAPGEF2 fusion protein Ag35288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rap guanine nucleotide exchange factor (GEF) 2
Synonyms PDZGEF1, PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313
Calculated Molecular Weight167kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberNM_014247.2
Gene Symbol RAPGEF2
Gene ID (NCBI) 9693
RRIDAB_3669936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9Y4G8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAPGEF2 antibody 31299-1-APDownload protocol
IF protocol for RAPGEF2 antibody 31299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols