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验证数据展示

  • WB analysis using 31299-1-AP

    WB analysis using 31299-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31299-1-AP (RAPGEF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 31299-1-AP

    IF Staining of mouse kidney using 31299-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using RAPGEF2 antibody (31299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAPGEF2 Polyclonal antibody

RAPGEF2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 31299-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

PDZGEF1, PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31299-1-AP targets RAPGEF2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAPGEF2 fusion protein Ag35288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rap guanine nucleotide exchange factor (GEF) 2
别名 PDZGEF1, PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313
计算分子量167kDa
观测分子量200 kDa
GenBank蛋白编号NM_014247.2
基因名称 RAPGEF2
Gene ID (NCBI) 9693
RRIDAB_3669936
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y4G8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAPGEF2 antibody 31299-1-APDownload protocol
IF protocol for RAPGEF2 antibody 31299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols