验证数据展示
经过测试的应用
Positive WB detected in | CHO cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse testis tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12572-1-AP targets EPAC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse, rat, hamster |
Immunogen | EPAC1 fusion protein Ag3240 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Rap guanine nucleotide exchange factor (GEF) 3 |
Synonyms | RAPGEF3, RAPGEF3, EPAC1, EPAC 1, EPAC, CGEF1 |
Calculated Molecular Weight | 881 aa, 99 kDa |
Observed Molecular Weight | 99-104 kDa |
GenBank Accession Number | BC017728 |
Gene Symbol | EPAC1/RAPGEF3 |
Gene ID (NCBI) | 10411 |
RRID | AB_11232407 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95398 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for EPAC1 antibody 12572-1-AP | Download protocol |
IHC protocol for EPAC1 antibody 12572-1-AP | Download protocol |
IF protocol for EPAC1 antibody 12572-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |