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验证数据展示

  • WB analysis of CHO using 12572-1-AP

    WB analysis of CHO using 12572-1-AP

    CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 12572-1-AP

    IHC staining of human stomach cancer using 12572-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 12572-1-AP

    IHC staining of human stomach cancer using 12572-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 12572-1-AP

    IF Staining of mouse testis using 12572-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 12572-1-AP

    WB analysis using 12572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

EPAC1 Polyclonal antibody

EPAC1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 12572-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, hamster

应用

WB, IHC, IF-P, ELISA

别名

RAPGEF3, RAPGEF3, EPAC1, EPAC 1, EPAC, CGEF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCHO cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12572-1-AP targets EPAC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, hamster
免疫原 EPAC1 fusion protein Ag3240 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rap guanine nucleotide exchange factor (GEF) 3
别名 RAPGEF3, RAPGEF3, EPAC1, EPAC 1, EPAC, CGEF1
计算分子量 881 aa, 99 kDa
观测分子量 99-104 kDa
GenBank蛋白编号BC017728
基因名称 EPAC1/RAPGEF3
Gene ID (NCBI) 10411
RRIDAB_11232407
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95398
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).

实验方案

Product Specific Protocols
WB protocol for EPAC1 antibody 12572-1-APDownload protocol
IHC protocol for EPAC1 antibody 12572-1-APDownload protocol
IF protocol for EPAC1 antibody 12572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols