验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue, HepG2 cells |
Positive IP detected in | mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
19103-1-AP targets EPAC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | rat |
免疫原 | EPAC2 fusion protein Ag5186 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Rap guanine nucleotide exchange factor (GEF) 4 |
别名 | CAMP GEFII, CGEF2, EPAC 2, EPAC2, Nbla00496, RAPGEF4 |
计算分子量 | 116 kDa |
观测分子量 | 110 kDa |
GenBank蛋白编号 | BC024004 |
基因名称 | EPAC2/RAPGEF4 |
Gene ID (NCBI) | 11069 |
RRID | AB_10597393 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8WZA2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for EPAC2 antibody 19103-1-AP | Download protocol |
IP protocol for EPAC2 antibody 19103-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cell Mol Med Differential changes in cyclic adenosine 3'-5' monophosphate (cAMP) effectors and major Ca2+ handling proteins during diabetic cardiomyopathy | ||
J Diabetes Investig Epac2 activation mediates glucagon-induced glucogenesis in primary rat hepatocytes |