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RAPGEF5 Polyclonal antibody

RAPGEF5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12556-1-AP

Print datasheet

Synonyms

GFR, KIAA0277, M Ras regulated Rap GEF, MR GEF, MRGEF, RAPGEF5, Related to Epac, REPAC

验证数据展示

  • WB analysis of human brain using 12556-1-AP

    WB analysis of human brain using 12556-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, K-562 cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12556-1-AP targets RAPGEF5 in WB, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RAPGEF5 fusion protein Ag3229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rap guanine nucleotide exchange factor (GEF) 5
Synonyms GFR, KIAA0277, M Ras regulated Rap GEF, MR GEF, MRGEF, RAPGEF5, Related to Epac, REPAC
Calculated Molecular Weight68 kDa
Observed Molecular Weight 68-75 kDa
GenBank Accession NumberBC039203
Gene Symbol RAPGEF5
Gene ID (NCBI) 9771
RRIDAB_2177476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92565
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAPGEF5 antibody 12556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,RIP

Redox Biol

CircRAPGEF5 interacts with RBFOX2 to confer ferroptosis resistance by modulating alternative splicing of TFRC in endometrial cancer

Authors - Jun Zhang
View Article