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验证数据展示

  • WB analysis of human brain using 21746-1-AP

    WB analysis of human brain using 21746-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21746-1-AP (RAPGEFL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21746-1-AP

    IF Staining of HepG2 using 21746-1-AP

    Immunofluorescent analysis of HepG2 cells, using RAPGEFL1 antibody 21746-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

RAPGEFL1 Polyclonal antibody

RAPGEFL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21746-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Link GEFII, RAPGEFL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse kidney tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21746-1-AP targets RAPGEFL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAPGEFL1 fusion protein Ag16506 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rap guanine nucleotide exchange factor (GEF)-like 1
别名 Link GEFII, RAPGEFL1
计算分子量 662 aa, 73 kDa
观测分子量 73 kDa, 59 kDa
GenBank蛋白编号BC115374
基因名称 RAPGEFL1
Gene ID (NCBI) 51195
RRIDAB_10733650
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHV5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAPGEFL1 antibody 21746-1-APDownload protocol
IF protocol for RAPGEFL1 antibody 21746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols