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RAPGEFL1 Polyclonal antibody

RAPGEFL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21746-1-AP

Print datasheet

Synonyms

Link GEFII, RAPGEFL1

验证数据展示

  • WB analysis of human brain using 21746-1-AP

    WB analysis of human brain using 21746-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21746-1-AP (RAPGEFL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21746-1-AP

    IF Staining of HepG2 using 21746-1-AP

    Immunofluorescent analysis of HepG2 cells, using RAPGEFL1 antibody 21746-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse kidney tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21746-1-AP is RAPGEFL1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAPGEFL1 fusion protein Ag16506 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rap guanine nucleotide exchange factor (GEF)-like 1
Synonyms Link GEFII, RAPGEFL1
Calculated Molecular Weight 662 aa, 73 kDa
Observed Molecular Weight 73 kDa, 59 kDa
GenBank Accession NumberBC115374
Gene Symbol RAPGEFL1
Gene ID (NCBI) 51195
RRIDAB_10733650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHV5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAPGEFL1 antibody 21746-1-APDownload protocol
IF protocol for RAPGEFL1 antibody 21746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols