• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of SKOV-3 using 25750-1-AP

    WB analysis of SKOV-3 using 25750-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25750-1-AP (RAPH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25750-1-AP

    IF Staining of HepG2 using 25750-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAPH1 antibody (25750-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

RAPH1 Polyclonal antibody

RAPH1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25750-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Lamellipodin, KIAA1681, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein, ALS2CR9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inSKOV-3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25750-1-AP targets RAPH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RAPH1 fusion protein Ag22705 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
别名 Lamellipodin, KIAA1681, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein, ALS2CR9
计算分子量 1250 aa, 135 kDa
观测分子量 67 kDa
GenBank蛋白编号BC156922
基因名称 RAPH1
Gene ID (NCBI) 65059
RRIDAB_2880223
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70E73
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAPH1 encodes the lamellipodin protein (Lpd), which binds to Ena and VASPs to assist in actin cytoskeleton assembly; in this way, the gene plays an important role in cellular motility and lamelipodial protrusion. This protein has 9 isoforms producing different molecular weights of 135 kDa, 67 kDa, 70 kDa, 73 kDa.

实验方案

Product Specific Protocols
WB protocol for RAPH1 antibody 25750-1-APDownload protocol
IF protocol for RAPH1 antibody 25750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols