验证数据展示
经过测试的应用
| Positive WB detected in | Sp2/0 cells |
| Positive IHC detected in | mouse colon tissue, mouse bladder tissue, human pancreas cancer tissue, human renal cell carcinoma tissue, mouse brain tissue, rat bladder tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
14013-1-AP targets RARB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | RARB fusion protein Ag5119 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoic acid receptor, beta |
| 别名 | Retinoic acid receptor beta, RAR-epsilon, RAR-beta, RAR beta, NR1B2 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 37-51 kDa |
| GenBank蛋白编号 | BC060794 |
| 基因名称 | RARB |
| Gene ID (NCBI) | 5915 |
| RRID | AB_2177765 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10826 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PMID:12554770). They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. RARB is one isotypes of RARs (PMID:12834868).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RARB antibody 14013-1-AP | Download protocol |
| IHC protocol for RARB antibody 14013-1-AP | Download protocol |
| IF protocol for RARB antibody 14013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Antiviral Res Retinoic acid receptor β, a potential therapeutic target in the inhibition of adenovirus replication.
| ||
