验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, U-251 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RARS1 Ready-To-Use IHC Kit. RARS1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
产品信息
27344-1-AP targets RARS in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RARS fusion protein Ag26390 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | arginyl-tRNA synthetase |
| 别名 | Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS |
| 计算分子量 | 75 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC000528 |
| 基因名称 | RARS |
| Gene ID (NCBI) | 5917 |
| RRID | AB_2880849 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54136 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RARS antibody 27344-1-AP | Download protocol |
| IHC protocol for RARS antibody 27344-1-AP | Download protocol |
| IP protocol for RARS antibody 27344-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants
| ||
bioRxiv Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells
| ||
Sci Adv Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells
|
