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- KD/KO Validated
RARS Polyclonal antibody
RARS Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, U-251 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RARS1 Ready-To-Use IHC Kit. RARS1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
产品信息
The immunogen of 27344-1-AP is RARS Fusion Protein expressed in E. coli.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | RARS fusion protein Ag26390 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | arginyl-tRNA synthetase |
| Synonyms | Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC000528 |
| Gene Symbol | RARS |
| Gene ID (NCBI) | 5917 |
| RRID | AB_2880849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54136 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RARS antibody 27344-1-AP | Download protocol |
| IHC protocol for RARS antibody 27344-1-AP | Download protocol |
| IP protocol for RARS antibody 27344-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants
| ||
bioRxiv Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells
| ||
Sci Adv Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells
|
