• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

RARS Monoclonal antibody, PBS Only

RARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8G4

Cat No : 66815-1-PBS

Print datasheet

Synonyms

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

验证数据展示

  • WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

    WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

  • WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

    WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

    WB analysis of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

    WB result of RARS antibody (66815-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

    IF Staining of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RARS antibody (66815-1-Ig, Clone: 1C8G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RARS1 Ready-To-Use IHC Kit. RARS1 primary antibody included.

产品信息

66815-1-PBS targets RARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RARS fusion protein Ag26399 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name arginyl-tRNA synthetase
Synonyms Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC000528
Gene Symbol RARS
Gene ID (NCBI) 5917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54136
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.