验证数据展示
经过测试的应用
| Positive WB detected in | 3T3-L1 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
27835-1-AP targets RASA2/3 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Immunogen | RASA2/3 fusion protein Ag27152 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RAS p21 protein activator 3 |
| Synonyms | GAP1(IP4BP), GAP1IP4BP, GAPIII, Ins P4 binding protein, RAS p21 protein activator 3, RASA2/3, RASA3 |
| Calculated Molecular Weight | 834 aa, 96 kDa |
| Observed Molecular Weight | 96 kDa |
| GenBank Accession Number | BC038456 |
| Gene Symbol | RASA3 |
| Gene ID (NCBI) | 22821 |
| RRID | AB_2880988 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14644 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rasa3 is a GTPase activating protein of the GAP1 family which targets R-Ras and Rap1. While R-Ras has been extensively studied due to its involvement in cancer, Rap1 has recently attracted a lot of attention due to its central role in development and morphogenesis of higher organisms, especially in the cardiovasculature. This antibody can react with RASA2 and RASA3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RASA2/3 antibody 27835-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arterioscler Thromb Vasc Biol Lipid Receptor GPR31 (G-Protein-Coupled Receptor 31) Regulates Platelet Reactivity and Thrombosis Without Affecting Hemostasis. | ||
