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  • Featured Product
  • KD/KO Validated

RASAL2 Polyclonal antibody

RASAL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 22140-1-AP

Print datasheet

Synonyms

nGAP, RAS protein activator like 2, RASAL2

验证数据展示

  • WB analysis of HeLa using 22140-1-AP

    WB analysis of HeLa using 22140-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 22140-1-AP

    IF Staining of HeLa using 22140-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22140-1-AP targets RASAL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen RASAL2 fusion protein Ag17555 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS protein activator like 2
Synonyms nGAP, RAS protein activator like 2, RASAL2
Calculated Molecular Weight 1262 aa, 142 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC110611
Gene Symbol RASAL2
Gene ID (NCBI) 9462
RRIDAB_2879005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASAL2 (Ras protein activator like 2), also known as nGAP, is a Ras GTPase-activating protein, containing a GRD domain and a coiled-coil structure at the C terminus. It is able to complement the defective RasGAP function in a yeast system. RASAL2 is highly homologous to Ras-specific GTPase activating proteins (RasGAPs). Two transcript variants exist for RASAL2 due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for RASAL2 antibody 22140-1-APDownload protocol
IF protocol for RASAL2 antibody 22140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Metab

Rasal2 deficiency reduces adipogenesis and occurrence of obesity-related disorders.

Authors - Xiaoqiang Zhu
  • KD Validated
View Article
human,mouseWB,IHC,IF

J Clin Transl Hepatol

RASAL2 Deficiency Attenuates Hepatic Steatosis by Promoting Hepatic VLDL Secretion via the AKT/TET1/MTTP Axis

Authors - Hao Ding
  • KD Validated
View Article
humanWB,IP,IHC,IF

Int J Biol Sci

RASAL2 mediated the enhancement of YAP1/TIAM1 signaling promotes malignant phenotypes of pancreatic ductal adenocarcinoma

Authors - Yangyang Yue
  • KD Validated
View Article
humanIF

Transl Res

RAS protein activator-like 2 (RASAL2) initiates peritubular capillary rarefaction in hypoxic renal interstitial fibrosis

Authors - Yu Zhang
View Article