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  • Featured Product
  • KD/KO Validated

RASEF Polyclonal antibody

RASEF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11569-1-AP

Print datasheet

Synonyms

Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45

验证数据展示

  • WB analysis of mouse testis using 11569-1-AP

    WB analysis of mouse testis using 11569-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11569-1-AP

    IHC staining of mouse brain using 11569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASEF antibody included
  • IHC staining of mouse brain using 11569-1-AP

    IHC staining of mouse brain using 11569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASEF antibody included

经过测试的应用

Positive WB detected inmouse testis tissue, mouse heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASEF Ready-To-Use IHC Kit. RASEF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11569-1-AP targets RASEF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RASEF fusion protein Ag2149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS and EF-hand domain containing
Synonyms Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45
Calculated Molecular Weight 740 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2238112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZ41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RASEF antibody 11569-1-APDownload protocol
IHC protocol for RASEF antibody 11569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Mol Cancer Res

RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.

Authors - Oshita Hideto H
  • KD Validated
View Article
humanIHC

Int J Clin Exp Pathol

High RASEF expression is associated with a significantly better prognosis in colorectal cancer

Authors - Xin Yu
View Article