验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26997-1-AP targets RASGRP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | RASGRP1 fusion protein Ag25725 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAS guanyl releasing protein 1 (calcium and DAG-regulated) |
别名 | Ras guanyl releasing protein, hRasGRP1, CalDAG-GEFII, CALDAG GEFII, CALDAG GEFI |
计算分子量 | 797 aa, 90 kDa |
观测分子量 | 85-90 kDa |
GenBank蛋白编号 | BC109297 |
基因名称 | RASGRP1 |
Gene ID (NCBI) | 10125 |
RRID | AB_3669579 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O95267 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RASGRP1 (RAS guanyl-releasing protein 1) is a Ras guanine nucleotide exchange factor, and an essential regulator of lymphocyte receptor signaling (PMID: 33065764). RasGRP1 is a bifunctional regulator that promotes acute inflammation and inhibits inflammation-associated cancer (PMID: 36385095). RasGRP1, a downstream target gene of VEGF, regulates the development, homeostasis, and differentiation of T cells (PMID: 37429143).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RASGRP1 antibody 26997-1-AP | Download protocol |
IF protocol for RASGRP1 antibody 26997-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |