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RASSF7 Polyclonal antibody

RASSF7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21753-1-AP

Print datasheet

Synonyms

C11orf13, HRAS1, HRC1, RASSF7

验证数据展示

  • WB analysis using 21753-1-AP

    WB analysis using 21753-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21753-1-AP

    IHC staining of human lung cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 21753-1-AP

    IHC staining of human lung cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, HepG2 cells, PC-12 cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21753-1-AP targets RASSF7 in WB, IHC, ELISA applications and shows reactivity with human, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, Rat
Immunogen RASSF7 fusion protein Ag16123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras association (RalGDS/AF-6) domain family (N-terminal) member 7
Synonyms C11orf13, HRAS1, HRC1, RASSF7
Calculated Molecular Weight 373 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC106921
Gene Symbol RASSF7
Gene ID (NCBI) 8045
RRIDAB_10858387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02833
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASSF7, previously known as HRC1 (HRAS1 cluster 1) and C11orf13, is a member of the N-terminal RASSF family. RASSF7 protein localises to the centrosome and plays a key role in mitosis. RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. RASSF7 has three isoforms with the MW 40 kDa, 36 kDa and 35 kDa.

实验方案

Product Specific Protocols
WB protocol for RASSF7 antibody 21753-1-APDownload protocol
IHC protocol for RASSF7 antibody 21753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols