RB1 Polyclonal antibody

RB1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25628-1-AP

Print datasheet

Synonyms

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1



经过测试的应用

Positive WB detected inJurkat cells, A431 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25628-1-AP targets RB1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IP, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RB1 fusion protein Ag22578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoblastoma 1
Synonyms OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1
Calculated Molecular Weight 928 aa, 106 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC039060
Gene Symbol RB1
Gene ID (NCBI) 5925
RRIDAB_2880168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06400
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RB1 antibody 25628-1-APDownload protocol
IF protocol for RB1 antibody 25628-1-APDownload protocol
IP protocol for RB1 antibody 25628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Artif Cells Nanomed Biotechnol

Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.

Authors - Tianqi Li
humanWB

Oncol Rep

Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.

Authors - Da-Peng Yun
humanIF

Oncol Lett

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Authors - Kun Lu
humanWB

Cell Death Dis

MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.

Authors - Fengxia Chen
humanWB

Biomed Pharmacother

Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

Authors - Yudi Xiong