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验证数据展示

  • WB analysis using 30373-1-AP

    WB analysis using 30373-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30373-1-AP (RB1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30373-1-AP

    IHC staining of human ovary cancer using 30373-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30373-1-AP (RB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 30373-1-AP

    IHC staining of human ovary cancer using 30373-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30373-1-AP (RB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 30373-1-AP

    IF Staining of HepG2 using 30373-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RB1 antibody (30373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

RB1 Polyclonal antibody

RB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30373-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

pRb, pp110, p110-RB1 , p110-RB1, p105-Rb

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HT-1080 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30373-1-AP targets RB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoblastoma 1
Synonyms pRb, pp110, p110-RB1 , p110-RB1, p105-Rb
Calculated Molecular Weight 928 aa, 106 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC039060
Gene Symbol RB1
Gene ID (NCBI) 5925
RRIDAB_3086301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06400
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).

实验方案

Product Specific Protocols
WB protocol for RB1 antibody 30373-1-APDownload protocol
IHC protocol for RB1 antibody 30373-1-APDownload protocol
IF protocol for RB1 antibody 30373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols