• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30374-1-AP

    WB analysis using 30374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30374-1-AP (RB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30374-1-AP

    IHC staining of human ovary cancer using 30374-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30374-1-AP (RB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 30374-1-AP

    IHC staining of human ovary cancer using 30374-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30374-1-AP (RB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RB1 Polyclonal antibody

RB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30374-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

pRb, pp110, p110-RB1 , p110-RB1, p105-Rb

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30374-1-AP targets RB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinoblastoma 1
别名 pRb, pp110, p110-RB1 , p110-RB1, p105-Rb
计算分子量 928 aa, 106 kDa
观测分子量106-110 kDa
GenBank蛋白编号BC039060
基因名称 RB1
Gene ID (NCBI) 5925
RRIDAB_3086302
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06400
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).

实验方案

Product Specific Protocols
WB protocol for RB1 antibody 30374-1-APDownload protocol
IHC protocol for RB1 antibody 30374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols