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RBBP6 Polyclonal antibody

RBBP6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11882-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

p53-associated cellular protein of testis, P2PR, P2P R, MY038, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman placenta tissue, human rectal cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RBBP6 Ready-To-Use IHC Kit. RBBP6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11882-1-AP targets RBBP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RBBP6 fusion protein Ag2484 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoblastoma binding protein 6
Synonyms p53-associated cellular protein of testis, P2PR, P2P R, MY038, EC:2.3.2.27
Calculated Molecular Weight 1792 aa, 200 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC029352
Gene Symbol RBBP6
Gene ID (NCBI) 5930
RRIDAB_2177794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6E9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

实验方案

Product Specific Protocols
WB protocol for RBBP6 antibody 11882-1-APDownload protocol
IHC protocol for RBBP6 antibody 11882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Commun Biol

The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells

Authors - Christopher Noyes
  • KD Validated
humanWB

iScience

RRM2 promotes liver metastasis of pancreatic cancer by stabilizing YBX1 and activating the TGF-beta pathway

Authors - Zhouyuan Du
  • KD Validated
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