验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, COS-7 cells, HEK-293T cells, HeLa cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy RBBP7 Ready-To-Use IHC Kit. RBBP7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
20365-1-AP targets RbAp46 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoblastoma binding protein 7 |
| 别名 | RBBP7, Retinoblastoma-binding protein p46, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | NM_002893 |
| 基因名称 | RBBP7 |
| Gene ID (NCBI) | 5931 |
| RRID | AB_10697660 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16576 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IHC protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IF protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IP protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Gastroenterol Hepatol Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.
| ||
Oncogene HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer. | ||
Biochem Biophys Res Commun PAFAH1B2 is a HIF1a target gene and promotes metastasis in pancreatic cancer.
| ||
Oncol Lett RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma.
| ||
Oncogene ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression | ||
J Biol Chem Elexacaftor/VX-445-mediated CFTR interactome remodeling reveals differential correction driven by mutation-specific translational dynamics |
