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验证数据展示

  • WB analysis of A549 using 66015-1-Ig

    WB analysis of A549 using 66015-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66015-1-Ig (RBBP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66015-1-Ig

    IF Staining of HeLa using 66015-1-Ig

    Immunofluorescent analysis of Hela cells, using RBBP9 antibody 66015-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

  • IHC staining of human pancreas cancer using 66015-1-Ig

    IHC staining of human pancreas cancer using 66015-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66015-1-Ig

    IHC staining of human pancreas cancer using 66015-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBBP9 Monoclonal antibody

RBBP9 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66015-1-Ig

Print datasheet

CloneNo. 1H6D8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, ELISA

Synonyms

RBBP9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66015-1-Ig targets RBBP9 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RBBP9 fusion protein Ag17864 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 9
Synonyms RBBP9
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC015938
Gene Symbol RBBP9
Gene ID (NCBI) 10741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell–cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)

实验方案

Product Specific Protocols
WB protocol for RBBP9 antibody 66015-1-IgDownload protocol
IHC protocol for RBBP9 antibody 66015-1-IgDownload protocol
IF protocol for RBBP9 antibody 66015-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols