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- KD/KO Validated
RBCK1 Polyclonal antibody
RBCK1 Polyclonal Antibody for IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26367-1-AP targets RBCK1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IP, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | RBCK1 fusion protein Ag24405 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RanBP-type and C3HC4-type zinc finger containing 1 |
| Synonyms | C20orf18, HBV associated factor 4, HOIL 1, HOIL1, RBCK1, RBCK2, RING finger protein 54, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4 |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC015219 |
| Gene Symbol | RBCK1 |
| Gene ID (NCBI) | 10616 |
| RRID | AB_2880493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| IP protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis RBCK1 regulates the progression of ER-positive breast cancer through the HIF1α signaling | ||
Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis
| ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma | ||
Hum Cell Regulation of PTEN and ovarian cancer progression by an E3 ubiquitin ligase RBCK1.
| ||
Biochem Biophys Res Commun Mechanistic insights into the homo-dimerization of HOIL-1L and SHARPIN | ||
BMC Musculoskelet Disord Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China. |