验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, COLO 320 cells |
| Positive IP detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13354-1-AP targets p107 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, non-human primate |
| 免疫原 | p107 fusion protein Ag4146 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoblastoma-like 1 (p107) |
| 别名 | CP107, p107, PRB1, RBL1, retinoblastoma like 1 (p107), Retinoblastoma like protein 1 |
| 计算分子量 | 1068 aa, 119 kDa |
| 观测分子量 | 119 kDa |
| GenBank蛋白编号 | BC032247 |
| 基因名称 | p107 |
| Gene ID (NCBI) | 5933 |
| RRID | AB_2238024 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Retinoblastoma-like protein 1, also named RBL1, p107, is a member of E1A-binding proteins, which contains a A/B pocket, or pRb. Via this structure, RBL1 can bind to viral oncoproteins and other perteins containing LXCXE motif. It regulates cell proliferation via phosphorylation-sensitive interactions with E2F transcription factors and other proteins. Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.(uniprot). It has been reported there are two transcirpts variants of RBL1, which encode a 119-kd protein and a 68-kd protein(PMID:7490090).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for p107 antibody 13354-1-AP | Download protocol |
| IP protocol for p107 antibody 13354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun p107 mediated mitochondrial function controls muscle stem cell proliferative fates. | ||
Cancers (Basel) RBL1/p107 Expression Levels Are Modulated by Multiple Signaling Pathways. | ||
Front Cell Dev Biol miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis | ||
Oncotarget Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells.
| ||
Biochem Pharmacol Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms. | ||
Mol Pharm Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells. |
