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  • KD/KO Validated

RBM10 Polyclonal antibody

RBM10 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 14423-1-AP

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Synonyms

RNA binding protein 10, KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9



经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14423-1-AP targets RBM10 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RBM10 fusion protein Ag5788 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 10
Synonyms RNA binding protein 10, KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC024153
Gene Symbol RBM10
Gene ID (NCBI) 8241
RRIDAB_2175593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98175
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

实验方案

Product Specific Protocols
WB protocol for RBM10 antibody 14423-1-APDownload protocol
IF protocol for RBM10 antibody 14423-1-APDownload protocol
IP protocol for RBM10 antibody 14423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Proc Natl Acad Sci U S A

RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5

Authors - Hyemin Lee
  • KD Validated
humanWB

Oncogene

RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.

Authors - Ji Hoon Jung
  • KD Validated
humanWB

BMC Mol Biol

Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.

Authors - Leslie C Sutherland
  • KD Validated
humanWB

J Cell Mol Med

RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway.

Authors - Xin Jin
humanWB,CoIP

Exp Cell Res

RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities

Authors - Yueyang Li
  • KD Validated
humanWB,IHC,RIP

Cancer Cell Int

RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC

Authors - Shan Cong
  • KD Validated