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RBM12B Polyclonal antibody

RBM12B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17137-1-AP

Print datasheet

Synonyms

RBM12B, RNA binding motif protein 12B, RNA binding protein 12B

验证数据展示

  • WB analysis using 17137-1-AP

    WB analysis using 17137-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17137-1-AP (RBM12B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 17137-1-AP

    IF Staining of A431 using 17137-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM12B antibody (17137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human liver using 17137-1-AP

    IHC staining of human liver using 17137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17137-1-AP (RBM12B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17137-1-AP targets RBM12B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RBM12B fusion protein Ag10894 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 12B
Synonyms RBM12B, RNA binding motif protein 12B, RNA binding protein 12B
Calculated Molecular Weight 765 aa, 87 kDa, 118 kDa
Observed Molecular Weight118-130 kDa
GenBank Accession NumberBC039260
Gene Symbol RBM12B
Gene ID (NCBI) 389677
RRIDAB_2175746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXT5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM12B antibody 17137-1-APDownload protocol
IHC protocol for RBM12B antibody 17137-1-APDownload protocol
IF protocol for RBM12B antibody 17137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Stress granules regulate stress-induced paraspeckle assembly.

Authors - Haiyan An
View Article