验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10196-1-AP targets RBM14 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Immunogen | RBM14 fusion protein Ag0254 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RNA binding motif protein 14 |
| Synonyms | RNA-binding protein 14, RNA-binding motif protein 14, RNA binding protein 14, PSP2, Paraspeckle protein 2 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC000488 |
| Gene Symbol | RBM14 |
| Gene ID (NCBI) | 10432 |
| RRID | AB_2175748 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM14 is a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM14 antibody 10196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Exp Mol Med The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability | ||
Brain Res Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network. | ||
Transl Cancer Res Molecular mechanism of RBM14-mediated promotion of proliferation, migration, and invasion in osteosarcoma
| ||
Int J Biol Macromol Subcellular proteomics reveals the crosstalk between nucleocytoplasmic trafficking and the innate immune response to Senecavirus A infection | ||
J Virol RBM14 inhibits the replication of porcine epidemic diarrhea virus by recruiting p62 to degrade nucleocapsid protein through the activation of autophagy and interferon pathway |
