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Species Reactivity
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验证数据展示

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included

RBM15B Monoclonal antibody, PBS Only

RBM15B Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67506-1-PBS

产品说明书

CloneNo. 1C2C11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

OTT3, One-twenty two protein 3, HuOTT3, HUMAGCGB, HsOTT3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RBM15B Ready-To-Use IHC Kit. RBM15B primary antibody included.

产品信息

67506-1-PBS targets RBM15B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 RBM15B fusion protein Ag17853 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 15B
别名 OTT3, One-twenty two protein 3, HuOTT3, HUMAGCGB, HsOTT3
计算分子量 890 aa, 97 kDa
观测分子量 105 kDa
GenBank蛋白编号BC001367
基因名称 RBM15B
Gene ID (NCBI) 29890
RRIDAB_2882729
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NDT2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).