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RBM15B Monoclonal antibody, PBS Only

RBM15B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2C11

Cat no : 67506-1-PBS

Print datasheet

Synonyms

OTT3, One-twenty two protein 3, HuOTT3, HUMAGCGB, HsOTT3

验证数据展示

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
Planning an IHC experiment? We recommend our IHCeasy RBM15B Ready-To-Use IHC Kit. RBM15B primary antibody included.

产品信息

67506-1-PBS targets RBM15B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen RBM15B fusion protein Ag17853 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA binding motif protein 15B
Synonyms OTT3, One-twenty two protein 3, HuOTT3, HUMAGCGB, HsOTT3
Calculated Molecular Weight 890 aa, 97 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC001367
Gene Symbol RBM15B
Gene ID (NCBI) 29890
RRIDAB_2882729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NDT2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).