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验证数据展示

  • WB analysis using 13918-1-AP

    WB analysis using 13918-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13918-1-AP (RBM17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 13918-1-AP

    IP experiment of MCF-7 using 13918-1-AP

    IP result of anti-RBM17 (IP:13918-1-AP, 4ug; Detection:13918-1-AP 1:10000) with MCF-7 cells lysate 1000 ug.

  • WB analysis of MCF-7 using 13918-1-AP

    WB analysis of MCF-7 using 13918-1-AP

    WB result of RBM17 antibody (13918-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells.

  • IHC staining of human ovary cancer using 13918-1-AP

    IHC staining of human ovary cancer using 13918-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13918-1-AP (RBM17 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 13918-1-AP

    IF Staining of A549 using 13918-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM17 antibody (13918-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of SKOV-3 using 13918-1-AP

    IF Staining of SKOV-3 using 13918-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using RBM17 antibody (13918-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

RBM17 Polyclonal antibody

RBM17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13918-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, MCF-7 cells, A2780 cells, HeLa cells, PC-3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells, A549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13918-1-AP targets RBM17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RBM17 fusion protein Ag4921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 17
别名 SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
计算分子量 401 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC039322
基因名称 RBM17
Gene ID (NCBI) 84991
RRIDAB_2238079
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96I25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

实验方案

Product Specific Protocols
WB protocol for RBM17 antibody 13918-1-APDownload protocol
IHC protocol for RBM17 antibody 13918-1-APDownload protocol
IF protocol for RBM17 antibody 13918-1-APDownload protocol
IP protocol for RBM17 antibody 13918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.

Authors - Sean-Patrick Riechers
View Article
humanWB

J Exp Clin Cancer Res

CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.

Authors - Dongni Chen
View Article