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  • Featured Product
  • KD/KO Validated

RBM17 Recombinant antibody, PBS Only

RBM17 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230125A3

Cat no : 82889-3-PBS

Print datasheet

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

验证数据展示

  • WB analysis using 82889-3-RR (same clone as 82889-3-PBS)

    WB analysis using 82889-3-RR (same clone as 82889-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82889-3-RR (RBM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    WB analysis of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    WB result of RBM17 antibody (82889-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    IP experiment of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    IP result of anti-RBM17 (IP:82889-3-RR, 4ug; Detection:82889-3-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82889-3-RR (same clone as 82889-3-PBS)

    IHC staining of human ovarian cancer using 82889-3-RR (same clone as 82889-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82889-3-RR (RBM17 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82889-3-RR

    Affinity and Kinetic Characterization of 82889-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-3-RR against Human RBM17 were performed. The affinity constant is 0.828 nM.

产品信息

82889-3-PBS targets RBM17 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RBM17 fusion protein Ag0985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RNA binding motif protein 17
Synonyms SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
Calculated Molecular Weight 45 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96I25
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.