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验证数据展示

  • WB analysis using 22103-1-AP

    WB analysis using 22103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 22103-1-AP

    IF Staining of U2OS using 22103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RBM22 Polyclonal antibody

RBM22 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22103-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

RNA-binding motif protein 22, RNA binding motif protein 22, Pre-mRNA-splicing factor RBM22, Pre mRNA splicing factor RBM22, fSAP47

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, Raji cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22103-1-AP targets RBM22 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RBM22 fusion protein Ag17315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 22
别名 RNA-binding motif protein 22, RNA binding motif protein 22, Pre-mRNA-splicing factor RBM22, Pre mRNA splicing factor RBM22, fSAP47
计算分子量 420 aa, 47 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC003402
基因名称 RBM22
Gene ID (NCBI) 55696
RRIDAB_2878996
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NW64
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)

实验方案

Product Specific Protocols
WB protocol for RBM22 antibody 22103-1-APDownload protocol
IF protocol for RBM22 antibody 22103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols