• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RBM22 Polyclonal antibody

RBM22 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22103-1-AP

Print datasheet

Synonyms

RNA-binding motif protein 22, RNA binding motif protein 22, Pre-mRNA-splicing factor RBM22, Pre mRNA splicing factor RBM22, fSAP47

验证数据展示

  • WB analysis using 22103-1-AP

    WB analysis using 22103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 22103-1-AP

    IF Staining of U2OS using 22103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inNIH/3T3 cells, Raji cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22103-1-AP targets RBM22 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RBM22 fusion protein Ag17315 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 22
Synonyms RNA-binding motif protein 22, RNA binding motif protein 22, Pre-mRNA-splicing factor RBM22, Pre mRNA splicing factor RBM22, fSAP47
Calculated Molecular Weight 420 aa, 47 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC003402
Gene Symbol RBM22
Gene ID (NCBI) 55696
RRIDAB_2878996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NW64
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)

实验方案

Product Specific Protocols
WB protocol for RBM22 antibody 22103-1-APDownload protocol
IF protocol for RBM22 antibody 22103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols