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验证数据展示

  • WB analysis using 84968-4-RR

    WB analysis using 84968-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84968-4-RR (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84968-4-RR

    IF Staining of HeLa using 84968-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (84968-4-RR, Clone: 242526B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84968-4-RR

    Affinity and Kinetic Characterization of 84968-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84968-4-RR against Human RBM25 were performed. The affinity constant is 2.38 nM.

RBM25 Recombinant antibody

RBM25 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84968-4-RR

产品说明书

CloneNo. 242526B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

242526B7, Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells，Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84968-4-RR targets RBM25 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RBM25 fusion protein Ag18244 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 25
别名 242526B7, Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25
计算分子量 843 aa, 100 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC113389
基因名称 RBM25
Gene ID (NCBI) 58517
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP49756
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

实验方案

Product Specific Protocols
WB protocol for RBM25 antibody 84968-4-RRDownload protocol
IF protocol for RBM25 antibody 84968-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols