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RBM25 Recombinant antibody

RBM25 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242526B7

Cat no : 84968-4-RR

Print datasheet

Synonyms

RED120, RBM 25, Protein S164, NET52, Arg/Glu/Asp-rich protein of 120 kDa

验证数据展示

  • WB analysis using 84968-4-RR

    WB analysis using 84968-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84968-4-RR (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84968-4-RR

    Affinity and Kinetic Characterization of 84968-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84968-4-RR against Human RBM25 were performed. The affinity constant is 2.38 nM.

经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells，Jurkat cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84968-4-RR targets RBM25 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RBM25 fusion protein Ag18244 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RNA binding motif protein 25
Synonyms RED120, RBM 25, Protein S164, NET52, Arg/Glu/Asp-rich protein of 120 kDa
Calculated Molecular Weight 843 aa, 100 kDa
Observed Molecular Weight110-120 kDa
GenBank Accession NumberBC113389
Gene Symbol RBM25
Gene ID (NCBI) 58517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49756
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

实验方案

Product Specific Protocols
WB protocol for RBM25 antibody 84968-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols