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验证数据展示

  • WB analysis using 31850-1-AP

    WB analysis using 31850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31850-1-AP (RBM27 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 31850-1-AP

    IF Staining of U2OS using 31850-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM27 antibody (31850-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

RBM27 Polyclonal antibody

RBM27 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31850-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RNA-Binding Protein 27, RNA Binding Motif Protein 27, KIAA1311

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Hela cell, jurkat cell, K-562 cell
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31850-1-AP targets RBM27 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RBM27 fusion protein Ag35822 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 27
别名 RNA-Binding Protein 27, RNA Binding Motif Protein 27, KIAA1311
计算分子量119 kDa
观测分子量140 kDa
GenBank蛋白编号NM_018989
基因名称 RBM27
Gene ID (NCBI) 54439
RRIDAB_3670129
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P2N5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM27 has been identified as a candidate autism gene, and is present in cytoplasmic and and nuclear speckles. RBM27 encodes a protein containing an RNA-Binding Motif, which confers its ability to bind RNA. Therefore, RBM27 is important in mRNA processing and the turnover of nuclear polyadenylate (pA+) RNA.

实验方案

Product Specific Protocols
WB protocol for RBM27 antibody 31850-1-APDownload protocol
IF protocol for RBM27 antibody 31850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols