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RBM41 Polyclonal antibody

RBM41 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15784-1-AP

Print datasheet

Synonyms

bB383K5.1, RBM41, RNA binding motif protein 41, RNA binding protein 41

验证数据展示

  • WB analysis using 15784-1-AP

    WB analysis using 15784-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15784-1-AP (RBM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 15784-1-AP

    IHC staining of human endometrial cancer using 15784-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15784-1-AP (RBM41 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human endometrial cancer using 15784-1-AP

    IHC staining of human endometrial cancer using 15784-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15784-1-AP (RBM41 antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HepG2 using 15784-1-AP

    IF Staining of HepG2 using 15784-1-AP

    Immunofluorescent analysis of HepG2 cells, using RBM41 antibody 15784-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, SGC-7901 cells, HepG2 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15784-1-AP targets RBM41 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RBM41 fusion protein Ag8483 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 41
Synonyms bB383K5.1, RBM41, RNA binding motif protein 41, RNA binding protein 41
Calculated Molecular Weight 413 aa, 47 kDa
Observed Molecular Weight 43-47 kDa
GenBank Accession NumberBC006986
Gene Symbol RBM41
Gene ID (NCBI) 55285
RRIDAB_2176112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IZ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM41 antibody 15784-1-APDownload protocol
IHC protocol for RBM41 antibody 15784-1-APDownload protocol
IF protocol for RBM41 antibody 15784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols