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  • Featured Product
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FOX2/RBM9 Polyclonal antibody

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12498-1-AP

Print datasheet

Synonyms

FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh

验证数据展示

  • WB analysis using 12498-1-AP

    WB analysis using 12498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 12498-1-AP

    WB analysis using 12498-1-AP

    WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells.

  • IHC staining of human breast cancer using 12498-1-AP

    IHC staining of human breast cancer using 12498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOX2/RBM9 antibody included
  • IF Staining of mouse brain using 12498-1-AP

    IF Staining of mouse brain using 12498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 12498-1-AP

    WB analysis using 12498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12498-1-AP

    IP experiment of HepG2 using 12498-1-AP

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug.

  • IP experiment of K-562 using 12498-1-AP

    IP experiment of K-562 using 12498-1-AP

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug.

  • IF Staining of HepG2 using 12498-1-AP

    IF Staining of HepG2 using 12498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, HEK-293 cells, Jurkat cells, NCCIT cells, HeLa cells, Neuro-2a cells
Positive IP detected inHepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, mouse brain tissue, human ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12498-1-AP targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen FOX2/RBM9 fusion protein Ag3168 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 9
Synonyms FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh
Calculated Molecular Weight 390 aa, 41 kDa
Observed Molecular Weight 50 and 60 kDa
GenBank Accession NumberBC013115
Gene Symbol RBM9
Gene ID (NCBI) 23543
RRIDAB_2877861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43251
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)

实验方案

Product Specific Protocols
WB protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
IHC protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
IF protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
IP protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Pharmacol

Integrated pan-cancer analysis reveals the immunological and prognostic potential of RBFOX2 in human tumors

Authors - Fengxian Huang
View Article
humanWB,IF,CoIP

Nat Cell Biol

RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation

Authors - Xiaoyang Dou
View Article
humanWB

iScience

Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis

Authors - Yingchao Wang
View Article