验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HepG2 cells, HEK-293 cells, Jurkat cells, NCCIT cells, HeLa cells, Neuro-2a cells |
| Positive IP detected in | HepG2 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human placenta tissue, mouse brain tissue, human ovary tumor tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12498-1-AP targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | FOX2/RBM9 fusion protein Ag3168 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RNA binding motif protein 9 |
| Synonyms | FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh |
| Calculated Molecular Weight | 390 aa, 41 kDa |
| Observed Molecular Weight | 50 and 60 kDa |
| GenBank Accession Number | BC013115 |
| Gene Symbol | RBM9 |
| Gene ID (NCBI) | 23543 |
| RRID | AB_2877861 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43251 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IHC protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IF protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IP protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Integrated pan-cancer analysis reveals the immunological and prognostic potential of RBFOX2 in human tumors | ||
Nat Cell Biol RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation | ||
iScience Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis |
