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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 83623-5-RR

    WB analysis using 83623-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83623-5-RR (RBMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83623-5-RR

    FC experiment of HepG2 using 83623-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBMS1 Recombinant antibody (83623-5-RR, Clone:240677D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83623-5-RR

    Affinity and Kinetic Characterization of 83623-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83623-5-RR against Human RBMS1 were performed. The affinity constant is 0.726 nM.

RBMS1 Recombinant antibody

RBMS1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83623-5-RR
CloneNo. 240677D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

MSSP 2, MSSP 1, MSSP, HCC 4, C2orf12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, DU145 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83623-5-RR targets RBMS1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RBMS1 fusion protein Ag1493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RNA binding motif, single stranded interacting protein 1
Synonyms MSSP 2, MSSP 1, MSSP, HCC 4, C2orf12
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC018951
Gene Symbol RBMS1
Gene ID (NCBI) 5937
RRIDAB_3671236
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP29558
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBMS1 (RNA binding motif single stranded interacting protein 1), also known as C2orf12. It is expected to be located in the nucleus, which is ubiquitinated in placenta and endometrium. Highest amounts are found in placenta, lung and heart. This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The molecular weight of RBMS1 is 45 kDa.

实验方案

Product Specific Protocols
WB protocol for RBMS1 antibody 83623-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols