验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, HEK-293 cells, human brain tissue, human liver tissue, rat liver tissue |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human liver tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22683-1-AP targets RBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | RBP1 fusion protein Ag18572 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | retinol binding protein 1, cellular |
别名 | CRBPI, CRBP1, CRBP I, CRABP I, Cellular retinol-binding protein I |
计算分子量 | 135 aa, 16 kDa |
观测分子量 | 14-21 kDa |
GenBank蛋白编号 | BC121052 |
基因名称 | RBP1 |
Gene ID (NCBI) | 5947 |
RRID | AB_11182381 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P09455 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RBP1 antibody 22683-1-AP | Download protocol |
IHC protocol for RBP1 antibody 22683-1-AP | Download protocol |
IF protocol for RBP1 antibody 22683-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Metabolism Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved. | ||
Transl Oncol Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. | ||
Brain Sci Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus. | ||
J Food Biochem Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice. | ||
Cell Death Dis The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.
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FASEB J The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation |