验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, Y79 cells |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14352-1-AP targets RBP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RBP3 fusion protein Ag5699 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinol binding protein 3, interstitial |
| 别名 | D10S64, D10S65, D10S66, IRBP, RBP3, RBPI, Retinol binding protein 3 |
| 计算分子量 | 135 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC039844 |
| 基因名称 | RBP3 |
| Gene ID (NCBI) | 5949 |
| RRID | AB_2096956 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10745 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBP3 (retinoid-binding protein 3), also called interstitial retinoid-binding protein (IRBP), is a large glycoprotein that is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix. It is known to bind retinoids and fatty acids. RBP3 migrates around 136-140 kDa in the western blot analysis. (PMID: 15928609)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBP3 antibody 14352-1-AP | Download protocol |
| IHC protocol for RBP3 antibody 14352-1-AP | Download protocol |
| IP protocol for RBP3 antibody 14352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med Identification of a novel cis-regulatory element essential for immune tolerance. | ||
Acta Biomater Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. | ||
Proc Natl Acad Sci U S A Development of a therapeutic anti-HtrA1 antibody and the identification of DKK3 as a pharmacodynamic biomarker in geographic atrophy. | ||
Diabetes Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-induced Retinal Dysfunction and Neurodegeneration through Rhodopsin. | ||
iScience A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. | ||
Sci Rep Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation |
