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验证数据展示

  • WB analysis using 29550-1-AP

    WB analysis using 29550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29550-1-AP (RBP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

RBP4 Polyclonal antibody

RBP4 Polyclonal Antibody for WB, ELISA
Cat No. 29550-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human plasma
Planning an IHC experiment? We recommend our IHCeasy RBP4 Ready-To-Use IHC Kit. RBP4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29550-1-AP targets RBP4 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol binding protein 4, plasma
别名 Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein
计算分子量 201 aa, 23 kDa
观测分子量23 kDa
GenBank蛋白编号BC020633
基因名称 RBP4
Gene ID (NCBI) 5950
RRIDAB_3669688
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02753
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBP4 (Retinol-binding protein 4), also known as PRO2222. It is expected to be located in the cytoplasm, which is expressed in renal tubules, pancreatic islets and hepatocytes with positivity in plasma. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post- translationally and results in defective delivery and supply to the epidermal cells. The molecular weight of RBP4 is 23 kDa.

实验方案

Product Specific Protocols
WB protocol for RBP4 antibody 29550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols