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RBP4 Polyclonal antibody

RBP4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29550-1-AP

Print datasheet

Synonyms

Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein

验证数据展示

  • WB analysis using 29550-1-AP

    WB analysis using 29550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29550-1-AP (RBP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, human plasma
Planning an IHC experiment? We recommend our IHCeasy RBP4 Ready-To-Use IHC Kit. RBP4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29550-1-AP targets RBP4 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinol binding protein 4, plasma
Synonyms Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC020633
Gene Symbol RBP4
Gene ID (NCBI) 5950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBP4 (Retinol-binding protein 4), also known as PRO2222. It is expected to be located in the cytoplasm, which is expressed in renal tubules, pancreatic islets and hepatocytes with positivity in plasma. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post- translationally and results in defective delivery and supply to the epidermal cells. The molecular weight of RBP4 is 23 kDa.

实验方案

Product Specific Protocols
WB protocol for RBP4 antibody 29550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols